Little is known about prehistoric elevation changes in Lake Okeechobee, south Florida, or the lake's response to changing sea level in the late Pleistocene and Holocene. Paleo-lake shorelines are typically studied through detailed examination of geomorphic remnants indicating lake level and some form of erosional or depositional activity. The very low relief around Lake Okeechobee provides a limited range of landforms, many of which have been altered by human activities. Nevertheless, satellite imagery and aerial photographs show distinct strandlines, some of which have been positively identified as paleo-lake shorelines in the field. Research into the age and origins of select shorelines is ongoing. An alternative approach has been taken in order to examine the entirety of this shallow but large (approximately 1890 square kilometers) body of water. Archaeological sites in the five counties surrounding Lake Okeechobee, as well as the modern lake shoreline, have been mapped on a GIS; their dates of occupation and types of culture are included. These peoples are known to have lived on or near their water and food sources--rivers, or the lake. Their occupations and likely responses to changing lake levels are discussed. Using these data, several maps showing portions of probable shorelines have been created. These are compared to photographs of actual landforms, to images showing known shorelines, and to suggested marine shorelines in south Florida. The relationship of the elevation of Lake Okeechobee to the ocean is examined, with some suggestions as to why lake levels changed. The ecological and geomorphic implications of fluctuating lake levels in this low-relief area are discussed.